Reacting interior protein of grain to fluoresce

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1997-01-04
Authors
Christenbury, Gerald
Buchele, Wesley
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Buchele, Wesley
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Iowa State University Research Foundation, Inc.
The Iowa State University Research Foundation (ISURF) seeks to protect the intellectual property (including new discoveries, technologies, or creative works) of the university's students & faculty which is continuously created through research and other undertakings. It seeks to manage, protect, and own all intellectual property on behalf of the university. The Iowa State University Research Foundation was created in 1938.
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Abstract

A method of measuring mechanical damage to a grain, such as corn, includes applying a reacting solution to a sample of corn. The solution contains a material, such as 8-anilino-1-naphthalene sulfonic acid, that reacts selectively with the damaged portion of the corn kernels, i.e., the internal protein that is exposed when the outer shell is penetrated or breached. Excess solution is washed off; and the kernels are dried for convenience of handling. The dried corn is ground to a uniform fineness and then spread over a predetermined area. What is inherently a three-dimensional or volume phenomena (damage) is converted to a two-dimensional or area measurement. The sample is exposed to ultraviolet light; and induced fluorescence is measured. The output of the measuring system is linearly related to, and thus a measure of the mechanical damage of the sample.

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