Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

7-1999

Journal or Book Title

Molecular Plant-Microbe Interactions

Volume

12

Issue

7

First Page

585

Last Page

591

DOI

10.1094/MPMI.1999.12.7.585

Abstract

A β-1,4-endoglucanase encoding cDNA (EGases, E.C. 3.2.1.4), named Mi-eng-1, was cloned from Meloidogyne incognita second-stage juveniles (J2). The deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. In M. incognita, the gene is transcribed in the migratory J2, in males, and in the sedentary adult females. In pre-parasitic J2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. The presence of β-1,4-endoglucanase transcripts in adult females could be related to the expression of the gene in esophageal glands at this stage. However, cellulase activity within the egg matrix of adult females suggests that the endoglucanase may also be synthesized in the rectal glands and involved in the extrusion of the eggs onto the root surface. The maximum identity of the predicted MI-ENG-1 catalytic domain with the recently cloned cyst nematode β-1,4-endoglucanases is 52.5%. In contrast to cyst nematodes, M. incognita pre-parasitic J2 were not found to express a β-1,4-endoglucanase devoid of a cellulose-binding domain.

Comments

This article is published as Rosso, Marie-Noëlle, Bruno Favery, Christine Piotte, Laury Arthaud, Jan M. De Boer, Richard S. Hussey, Jaap Bakker, Thomas J. Baum, and Pierre Abad. "Isolation of a cDNA encoding a β-1, 4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism." Molecular Plant-Microbe Interactions 12, no. 7 (1999): 585-591, doi: 10.1094/MPMI.1999.12.7.585.

Rights

This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1999.

Language

en

File Format

application/pdf

Share

COinS