Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

6-2016

Journal or Book Title

Plant Physiology

Volume

171

Issue

2

First Page

760

Last Page

772

DOI

10.1104/pp.16.00172

Abstract

Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucumber mosaic virus have been shown to induce VIGS in maize (Zea mays). We describe here a new DNA-based VIGS system derived from Foxtail mosaic virus (FoMV), a monopartite virus that is able to establish systemic infection and silencing of endogenous maize genes homologous to gene fragments inserted into the FoMV genome. To demonstrate VIGS applications of this FoMV vector system, four genes, phytoene desaturase (functions in carotenoid biosynthesis), lesion mimic22 (encodes a key enzyme of the porphyrin pathway), iojap (functions in plastid development), and brown midrib3 (caffeic acid O-methyltransferase), were silenced and characterized in the sweet corn line Golden × Bantam. Furthermore, we demonstrate that the FoMV infectious clone establishes systemic infection in maize inbred lines, sorghum (Sorghum bicolor), and green foxtail (Setaria viridis), indicating the potential wide applications of this viral vector system for functional genomics studies in maize and other monocots.

Comments

This article is published as Mei, Yu, Chunquan Zhang, Bliss M. Kernodle, John H. Hill, and Steven A. Whitham. "A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize." Plant Physiology 171, no. 2 (2016): 760-772. doi: 10.1104/pp.16.00172. Posted with permission.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

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