Campus Units

Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

2011

Journal or Book Title

Biotechnology Letters

Volume

33

First Page

2217

Last Page

2223

DOI

10.1007/s10529-011-0682-9

Abstract

Analysis of an organism’s genetic diversity requires a method that gives reliable, reproducible results. Microsatellites are robust markers, however, detection of allele sizes can be difficult with some systems as well as consistency among laboratories. In this study, our two laboratories used 219 isolates of Phytophthora sojae to compare three microsatellite methods. Two capillary electrophoresis methods, the Applied Biosystems 3730 Genetic Analyzer and the CEQ 8000 Genetic Analysis system, detected an average of 2.4-fold more alleles compared to gel electrophoresis with a mean of 8.8 and 3.6 alleles per locus using capillary and gel methods, respectively. The two capillary methods were comparable, although allele sizes differed consistently by an average of 3.2 bp across isolates. Differences between capillary methods could be overcome if reference standard DNA genotypes are shared between collaborating laboratories.

Comments

This is an article from Biotechnology Letters 33 (2011): 2217, doi:10.1007/s10529-011-0682-9. Posted with permission.

Rights

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

Share

COinS