Degree Type

Dissertation

Date of Award

2008

Degree Name

Doctor of Philosophy

Department

Theses & dissertations (Interdisciplinary)

Major

Bioinformatics and Computational Biology

First Advisor

Robert L. Jernigan

Second Advisor

Zhijun Wu

Third Advisor

Vasant Honavar

Abstract

This PhD dissertation mainly focuses on the statistical analysis for the protein structure data. The first research project focuses on data mining and prediction for side chain orientation in protein structure. Through this study, we find that the general side chain orientation can be viewed as a manifestation of hydrophobic force. Along with this study, we also developed the software for visualizing the general side chain vector and applied statistical machine learning methods to fit several models for predicting general side chain orientation. In the second project, we studied the motion of partially assembled ribosome 30S subunit using the coarse-grained elastic network model. Besides our studies on ribosome motion, using 176 NMR structure ensembles, we applied principal component analysis to analyze the essential conformational changes to validate the motion generated by the elastic network model. Furthermore, we also studied the effects of different superposition methods on the correspondence between the conformational changes and the simulated motion. Principal component shaving method is often used to cluster gene with the similar gene expression pattern in micro-array data analysis. In the third research project, we applied this method to cluster the structures within a NMR structure ensemble and demonstrated that this method could be used to find the similar structure cluster.

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Aimin Yan

Language

en

Proquest ID

AAI3316183

OCLC Number

270709857

ISBN

9780549688143

File Format

application/pdf

File Size

150 pages

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