Interspecific hybridization in the genus Glycine is difficult and few successes have resulted from a multitude of attempts (Newell and Hymowitz, 1982, 1983). Because flowers of the perennial species are especially difficult to manipulate in the field or greenhouse, a study was undertaken to examine the potential of fertilizing these species in vitro, thereby partially alleviating mechanical damage to the ovulary and environmental stress. Initial studies were intended to develop in vitro procedures under which ovularies would survive and form viable embryos. Therefore, all flowers were self-fertilized.