We are using vertical polyacrylamide gel electrophoresis (Davis, 1964) and a staining system modified after Beauchamp and Fridovich (1971) to study superoxide dismutase polymorphisms in the subgenus soja. This staining system generates superoxide radical; hence, it is specific for SOD activity. We re-solve up to 9 SOD bands in dry or germinating soybean cotyledons, and in leaves.
Griffin, J. D. and Palmer, R. G.
"Research Notes : United States : Superoxide dismutase (SOD) in soybean,"
Soybean Genetics Newsletter: Vol. 11
, Article 34.
Available at: http://lib.dr.iastate.edu/soybeangenetics/vol11/iss1/34