We tested gradient PAGE gels for their ability to resolve isozymes with a specific SOD (EC 22.214.171.124) stain as well as with a nonspecific TO stain. In particular, we wanted to test gradient gels for use in scoring F2 seeds segregating for the cultivar-specific type 1 and type 3 TO zymogram patterns first reported by Gorman and Kiang (1977). TO electrophoretic bands are caused by any number of enzymes (particularly SOD) that prevent the spontaneous reduction of tetrazolium dyes in the electron-transfer staining systems used to detect dehydrogenases.
Kring, S. and Gorman, M. B.
"Research Notes : United States : Superoxide dismutase (SOD) and tetrazolium oxidase (TO) zymograms observed in gradient PAGE gels and preliminary inheritance data for type 3 zymograms,"
Soybean Genetics Newsletter: Vol. 14
, Article 72.
Available at: http://lib.dr.iastate.edu/soybeangenetics/vol14/iss1/72