Ethidium bromide (EB), an acridine compound which binds to nucleic acids, has been used as a mutagen in peanuts by Levy and Ashri (1975). Burton and Hanna (1976) induced cytoplasmic male sterile mutations in peari millet with EB and suggested that EB as a cytoplasmic mutagen might aid in the development of a cytoplasmic male sterile nuclear restorer system in other crops. The following i s a report on preliminary attempts to induce cytoplasmic male sterility in soybeans using seed treatments of ethidium bromide.
Burton, J. W.
"Research Notes: Induction of sterility in soybeans with ethidium bromide,"
Soybean Genetics Newsletter: Vol. 4
, Article 30.
Available at: http://lib.dr.iastate.edu/soybeangenetics/vol4/iss1/30