Irradiation treatment of seeds, pollen, or sporocytes has been used successfully to produce aneuploids in a number of genera. When Dr. E. G. Hammond had finished selecting M1 plants from his neutron irradiation experiment, I had the opportunity to go through his radiated material to select off-type and semi-sterile plants and to determine the kinds of aneuploids produced by radiation of soybeans. One- and two-seeded pods from remnant M1 plants were harvested, the M2 plants grown in the field, and the M3 progenies of M2 plants with more than 20% aborted pollen grains were checked for chromosome number and aberrations, using root tip squashes.
Sadanaga, K. and Grindeland, R.
"Research notes: Aneuploids and chromosome aberrations from irradiated soybeans,"
Soybean Genetics Newsletter: Vol. 6
, Article 21.
Available at: http://lib.dr.iastate.edu/soybeangenetics/vol6/iss1/21