In soybean plants carrying the gene T and having black or brown pigmentation of seed coats, there are numerous compounds that can be detected by thin layer chromatography (TLC). There are four spots (A, B, C and D) that appear to be related; they are yellow-orange under visible light and they fluoresce yellow-orange (duller than quercetin) under UV light after spray-ing with flavone reagent. Phenotypic positions of A and D on 2-way plates are given in Table 1. A is present in all material tested, but D varies in presence/absence (B and C also vary but have not been studied).
Buzzell, R. I. and Buttery, B. R.
"Research Notes : Inheritance of presence/absence of flavonoid compounds in soybean
Soybean Genetics Newsletter: Vol. 9
, Article 10.
Available at: http://lib.dr.iastate.edu/soybeangenetics/vol9/iss1/10