The methanogen electron carrier F420 was extracted from a methanogen cell culture, Methanobrevibacter ruminantium, and separated from other cell components by a single anion exchange chromatographic process. The extent of separation was determined spectroscopically. The fluorescence lifetime of F420 at pH 7.5 is ~4.2 nanoseconds.
Iowa State University
August 18, 2012
Ashby, K. D.; Kraus, George A.; Petrich, J. W.; Casey, T. A.; Rasmussen, M. A.; and Carpenter, Susan L., "Steady-State and Time-Resolved Spectroscopy of F420 Extracted from Methanogen Cells" (1999). Swine Research Report, 1998. Paper 55.