Placement of Intracoelomic Radiotransmitters and Silicone Passive Sampling Devices in Northern Leopard Frogs (Lithobates pipiens)

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2017-12-01
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Swanson, Jennifer
Pierce, Clay
Muths, Erin
Vandever, Mark
Zaffarano, Bianca
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Zaffarano, Bianca
Clinical Associate Professor Emeritus
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Pierce, Clay
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Veterinary Clinical Sciences
The mission of the Veterinary Clinical Sciences Department and the Veterinary Medical Center is to be strong academically, to provide outstanding services, and to conduct research in the multiple areas of Veterinary Clinical Sciences. Our goals are to teach students in the multiple disciplines of Veterinary Clinical Sciences, to provide excellent veterinary services to clients, and to generate and disseminate new knowledge in the areas of Veterinary Clinical Sciences. Our objectives are to provide a curriculum in the various aspects of Veterinary Clinical Sciences which ensures students acquire the skills and knowledge to be successful in their chosen careers. We also strive to maintain a caseload of sufficient size and diversity which insures a broad clinical experience for students, residents, and faculty. In addition, we aim to provide clinical veterinary services of the highest standards to animal owners and to referring veterinarians. And finally, we strive to provide an environment and opportunities which foster and encourage the generation and dissemination of new knowledge in many of the disciplines of Veterinary Clinical Sciences.
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Natural Resource Ecology and Management
The Department of Natural Resource Ecology and Management is dedicated to the understanding, effective management, and sustainable use of our renewable natural resources through the land-grant missions of teaching, research, and extension.
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Veterinary Clinical SciencesNatural Resource Ecology and Management
Abstract

Historically, wetland toxin exposure studies have relied on single time point samples from stationary sampling devices. Development of passive sampling devices (PSDs) that can be attached to individual animals within wetland habitats has greatly improved in recent years, presenting an innovative sampling technology that can potentially yield individual-specific, quantifiable data about chemical exposure. In this study, silicone based PSDs were attached to the ventral skin of 20 northern leopard frogs (Lithobates pipiens) with polypropylene sutures after radiotransmitters had been surgically implanted into the coleomic cavity. After a short recovery period, frogs were released back into the wetland habitat where they were acquired. The animals were located daily using radiotelemetry to assess how long PSDs would remain attached in the frogs' natural habitat. After one week, PSDs remained on 18 of the original 20 frogs. At two weeks, 17 frogs were recovered and no PSDs remained attached. Although valuable data can be obtained over a short time period, more research will be necessary to demonstrate the effectiveness of externally attaching silicone PSDs to northern leopard frogs for time periods longer than 1–2 weeks.

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This article is published as Yaw, Taylor J., Jennifer E. Swanson, Clay L. Pierce, Erin Muths, Kelly L. Smalling, Mark W. Vandever, and Bianca A. Zaffarano. "Placement of Intracoelomic Radiotransmitters and Silicone Passive Sampling Devices in Northern Leopard Frogs (Lithobates pipiens)." Journal of Herpetological Medicine and Surgery 27, no. 3-4 (2017): 111-115. DOI: 10.5818/16-10-090.1.

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