Veterinary Diagnostic and Production Animal Medicine, Veterinary Microbiology and Preventive Medicine, Genome Informatics Facility
Journal or Book Title
Frontiers in Microbiology
Campylobacter jejuni is a leading cause of human gastrointestinal disease and small ruminant abortions in the United States. The recent emergence of a highly virulent, tetracycline-resistant C. jejuni subsp. jejunisheep abortion clone (clone SA) in the United States, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organism. Pacific Biosciences' Single Molecule, Real-Time sequencing technology was used to explore the variation in the genome-wide methylation patterns of the abortifacient clone SA (IA3902) and phenotypically distinct gastrointestinal-specific C. jejuni strains (NCTC 11168 and 81-176). Several notable differences were discovered that distinguished the methylome of IA3902 from that of 11168 and 81-176: identification of motifs novel to IA3902, genome-specific hypo- and hypermethylated regions, strain level variability in genes methylated, and differences in the types of methylation motifs present in each strain. These observations suggest a possible role of methylation in the contrasting disease presentations of these three C. jejuni strains. In addition, the methylation profiles between IA3902 and a luxS mutant were explored to determine if variations in methylation patterns could be identified that might explain the role of LuxS-dependent methyl recycling in IA3902 abortifacient potential.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Kathy T. Mou, et al
Mou, Kathy T. Y.; Muppirala, Usha; Severin, Andrew J.; Clark, Tyson A.; Boitano, Matthew; and Plummer, Paul J., "A Comparative Analysis of Methylome Profiles of Campylobacter jejuni Sheep Abortion Isolate and Gastroenteric Strains Using PacBio Data" (2015). Veterinary Diagnostic and Production Animal Medicine Publications. 44.