Document Type

Article

Publication Date

11-2011

Journal or Book Title

Alcohol

Volume

45

Issue

7

First Page

673

Last Page

680

DOI

10.1016/j.alcohol.2010.11.001

Abstract

The effects of ethanol exposure on fetal lungs remain under investigation. Previously, we demonstrated that lambs exposed to ethanol during gestation had impaired expression of pulmonary surfactant protein A, a crucial component of lung immunity. In this study, we investigated the effects of in utero exposure to ethanol on maturation and immunity of the fetal lung. Pregnant ewes were surgically implanted with an abomasal cannula and administered 1 g ethanol/kg (n = 8) or water (n = 8) during the last trimester of pregnancy. Lambs were delivered prematurely or naturally. Neonatal lungs were assessed for maturation markers (hypoxia-inducible factor-1α [HIF-1α], HIF-2α, HIF-3α, vascular endothelial growth factor-A [VEGF-A], VEGFR-1, VEGFR-2, glycogen, and lung protein levels) and immunity (cytokines and chemokines). Preterm animals exposed to ethanol had significantly reduced VEGF-A mRNA (p = .066) and protein levels, HIF-1α (p = .055), HIF-2α (p = .019), VEGFR-1 (p = .088), and VEGFR-2 (p = .067) mRNA levels but no changes in HIF-3α mRNA. No significant changes occurred in full-term animals exposed to ethanol. Glycogen levels were significantly higher in preterm animals exposed to ethanol (p = .006) but not in full-term animals. Ethanol exposure was associated with significantly lower lung protein levels in preterm (p = .03) but not full-term animals. Preterm animals exposed to ethanol had significantly reduced TNF-α (p = .05), IL-10 (p = .03), chemokine (C-C motif) ligand 5 (CCL5) (p = .017), and monocyte chemotactic protein-1 (MCP-1) (p = .0004) mRNA. In full-term animals exposed to ethanol, the immune alterations were either sustained (TNF-α, p = .009; IL-10, P = .03) or returned to near baseline levels (CCL5 and MCP-1). The ethanol-mediated alterations in fetal lung maturation and immunity may explain the increased incidence of respiratory infections in neonates exposed to ethanol in utero.

Comments

This is an author's accepted manuscript of an article from Alcohol 45, no. 7 (November 2011): 673–680, doi:10.1016/j.alcohol.2010.11.001.

Copyright Owner

Elsevier, Inc.

Language

en

File Format

application/pdf

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