Campus Units

Veterinary Diagnostic and Production Animal Medicine, Veterinary Pathology

Document Type

Article

Publication Version

Published Version

Publication Date

9-2016

Journal or Book Title

Gene Reports

Volume

4

First Page

213

Last Page

217

DOI

10.1016/j.genrep.2016.06.004

Abstract

Transmissible spongiform encephalopathies (TSE) are caused by accumulation of a misfolded form of the prion protein (PrP). The normal cellular isoform of PrP is produced by the prion gene (PRNP) and is highly expressed in the central nervous system. Currently, there is an absence of information regarding the genetic sequence of alpaca PRNP and the potential susceptibility of this species to TSE. The objective of this study was to sequence the open reading frame of the alpaca prion gene and analyze this sequence for variation within the alpaca population and for homology to TSE-susceptible species. We sequenced the open reading frame of the prion gene of 40 alpacas of Huacaya or Suri descent. Length polymorphisms were identified within the sampled population. A subset (15%) of animals contained an additional 24 base pairs within the putative octapeptide repeat region. This polymorphism was independent of breed and sex. The majority (52.5%) of animals were heterozygous, possessing both longer and shorter alleles. Comparison with proven TSE-susceptible species (sheep, cattle, deer) revealed the following amino acid sequence variations: I6M, A16V, M17T, G92del, Q95_G96insG, N111S, R167K, N/T177S, I206V, S225Y, Y228S, Q230G, and L237del. Sequence alignment showed high homology compared to camel (> 95%), sheep (> 88%), cattle (> 87%) and deer (> 88%) PRNP sequence. This study demonstrates intraspecies variability within the PRNP open reading frame in alpacas and overall high sequence homology to TSE-susceptible species, providing foundational data for further research on the potential susceptibility of alpacas to TSE.

Comments

This article is published as Vermette, M. S., J. A. Schleining, J. J. Greenlee, and J. D. Smith. "Genetic variation of the prion protein gene (PRNP) in alpaca (Vicugna pacos)." Gene Reports 4 (2016): 213-217. doi: 10.1016/j.genrep.2016.06.004.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

Share

COinS