Campus Units

Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

1993

Journal or Book Title

Journal of Vacuum Science & Technology A

Volume

11

Issue

4

First Page

820

Last Page

823

DOI

10.1116/1.578311

Abstract

We have imaged gold‐labeled DNA molecules with the atomic force microscope(AFM). Circular plasmid DNA was labeled at internal positions by nick‐translation using biotinylated dUTP. For visualization, the biotinylated DNA was linked to streptavidin‐coated colloidal gold spheres (nominally 5 nm diam) prior to AFM imaging. Reproducible images of the labeled DNA were obtained both in dry air and under propanol. Height measurements of the DNA and colloidal gold made under both conditions are presented. The stability of the DNA‐streptavidin colloidal gold complexes observed even under propanol suggests that this labeling procedure could be exploited to map regions of interest in chromosomal DNA.

Comments

This article is from Journal of Vacuum Science & Technology A 11 (1993): 820, doi: 10.1116/1.578311. Posted with permission.

Copyright Owner

American Vacuum Society

Language

en

File Format

application/pdf

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