Campus Units

Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

1993

Journal or Book Title

Nucleic Acids Research

Volume

21

Issue

1

First Page

99

Last Page

103

DOI

10.1093/nar/21.1.99

Abstract

The atomic force microscope (AFM;1) can image DNA and RNA in air and under solutions at resolution comparable to that obtained by electron microscopy (EM) (2–7). We have developed a method for depositing and imaging linear DNA molecules to which 5nm gold spheres have been attached. The gold spheres facilitate orientation of the DNA molecules on the mica surface to which they are adsorbed and are potentially useful as internal height standards and as high resolution gene or sequence specific tags. We show that by modulating their adhesion to the mica surface, the gold spheres can be moved with some degree of control with the scanning tip.

Comments

This article is from Nucleic Acids Research 21 (1993): 99, doi: 10.1093/nar/21.1.99. Posted with permission.

Copyright Owner

Oxford University Press

Language

en

File Format

application/pdf

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