Campus Units

Zoology, Molecular, Cellular and Developmental Biology, Genetics

Document Type

Article

Publication Version

Published Version

Publication Date

1994

Journal or Book Title

Nucleic Acids Research

Volume

22

Issue

13

First Page

2479

Last Page

2489

DOI

10.1093/nar/22.13.2479

Abstract

We have postulated that chromosomal replication origin regions in eukaryotes have in common clusters of certain modular sequence elements (Benbow, Zhao, and Larson, BioEssays 14, 661–670, 1992). In this study, computer analyses of DNA sequences from six origin regions showed that each contained one or more potential initiation regions consisting of a putative DUE (DNA unwinding element) aligned with clusters of SAR (scaffold associated region), and ARS (autonomously replicating sequence) consensus sequences, and pyrimidine tracts. The replication origins analyzed were from the following loci: Tetrahymena thermophila macronuclear rDNA gene, Chinese hamster ovary dihydrofolate reductase amplicon, human c- myc protooncogene, chicken histone H5 gene, Drosophila melanogaster chorion gene cluster on the third chromosome, and Chinese hamster ovary rhodopsin gene. The locations of putative initiation regions identified by the computer analyses were compared with published data obtained using diverse methods to map initiation sites. For at least four loci, the potential initiation regions identified by sequence analysis aligned with previously mapped initiation events. A consensus DNA sequence, WAWTTDDWWWDHWGWHMAWTT, was found within the potential initiation regions in every case. An additional 35 kb of combined flanking sequences from the six loci were also analyzed, but no additional copies of this consensus sequence were found.

Comments

This article is from Nucleic Acids Research 22 (1994): 2479, doi: 10.1093/nar/22.13.2479. Posted with permission.

Copyright Owner

Oxford University Press

Language

en

File Format

application/pdf

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