Campus Units

Mathematics, Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

1996

Journal or Book Title

Journal of Vacuum Science & Technology B

Volume

14

Issue

2

First Page

1405

Last Page

1409

DOI

10.1116/1.589108

Abstract

Scanning force microscopy (SFM) reveals surface topography by scanning a sharp tip in close proximity to the sample. Due to tip–sample interaction, artificial broadening of the real surface structure with the tip geometry occurs. One approach for image reconstruction is the use of calibration standards, preferably in the size range of the samples. In the present study, an image reconstruction method based on colloidal gold as a geometric standard was used to reconstruct SFM images of biomolecules. Sample and calibration standard size were in the nanometer range, and the standards were coadsorbed with the specimen. Raw and reconstructed images of the biomolecules were compared, and the reconstruction was characterized by difference images as well as determination of the difference volume. The application of image reconstruction based on colloidal gold as a calibration standard for SFM of biomolecules is discussed.

Comments

This article is from Journal of Vacuum Science & Technology B 14 (1996): 1405, doi: 10.1116/1.589108. Posted with permission.

Copyright Owner

American Vacuum Society

Language

en

File Format

application/pdf

Share

COinS