Campus Units

Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

12-1994

Journal or Book Title

Neuron

Volume

13

Issue

6

First Page

1275

Last Page

1280

DOI

10.1016/0896-6273(94)90414-6

Abstract

Studies using biophysical techniques suggest a highly structured organization of calcium channels at the presynaptic transmitter release face (Llinás et al., 1981; Stanley, 1993), but it has not as yet proved possible to localize identified channels at the required nanometer level of resolution. We have used atomic force microscopy on the calyx-type nerve terminal of the chick ciliary ganglion to localize single calcium channels tagged via biotinylated ω-conotoxin GVIA to avidin-coated 30 nm gold particles. Calcium channels were in low (modal value approximately ⩽ 1 per μm2) and high (modal value55 per μm2) density areas and exhibited a prominent interchannel spacing of 40 nm, indicating an intermolecular linkage. Particles were observed in clusters and short linear or parallel linear arrays, groupings that may reflect calcium channel organization at the transmitter release site.

Comments

This article is from Neuron 13 (1994): 1275, doi: 10.1016/0896-6273(94)90414-6.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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