Campus Units

Zoology, Mathematics

Document Type

Article

Publication Version

Published Version

Publication Date

1996

Journal or Book Title

Journal of Vacuum Science & Technology B

Volume

14

Issue

2

First Page

1413

Last Page

1417

DOI

10.1116/1.589110

Abstract

A fundamental problem in atomic force microscopy (AFM) image interpretation is distinguishing features arising from tip geometry from true molecular detail. In this study, a novel 4‐stranded form of DNA (the ‘‘G wire’’) was coadsorbed with 7.6‐nm‐diam colloidal gold probe calibration standards and examined by AFM. After the probe apices were reconstructed from AFM images of the standards, the artificial broadening of the coadsorbed G‐wire DNA was removed, resulting in more reliable image interpretation. Using simple geometric models, a favorable comparison between observed and modeled G‐wire cross sections suggests that reconstructions removed about 25% of the tip‐broadened AFMimage in these studies.

Comments

This article is from Journal of Vacuum Science & Technology B 14 (1996): 1413, doi: 10.1116/1.589110. Posted with permission.

Copyright Owner

American Vacuum Society

Language

en

File Format

application/pdf

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