Campus Units

Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

1993

Journal or Book Title

Journal of Cell Science

Volume

104

Issue

2

First Page

427

Last Page

432

Abstract

The atomic force microscope (AFM) was used to directly image hippocampal neurons and glia. Using chemically fixed and living cells it was possible to reconstruct three-dimensional cell structure and detect sub-cellular features such as the nucleus, mitochondria and filaments. By repeatedly scanning a single living cell we observed the movement of filaments beneath the cell membrane. Furthermore, by controlling the force applied to the scanning tip, nanosurgery was performed to selectively remove cells from the culture substratum. Thus, the atomic force microscope offers the opportunity to gain three-dimensional information about living cells and to observe the behavior of cellular components by imaging through the intact cell membrane.

Comments

This is an article from Journal of Cell Science 104 (1993): 427. Posted with permission.

Copyright Owner

The Company of Biologists Limited

Language

en

File Format

application/pdf

Share

COinS