Campus Units

Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

9-2001

Journal or Book Title

Experimental and Molecular Medicine

Volume

33

Issue

3

First Page

174

Last Page

178

DOI

10.1038/emm.2001.30

Abstract

Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection, owing to the substantial overlap of the time-courses for both effects. Therefore, to determine transfection-induced cell toxicity, the mammalian expression vector pEGFP-N1 (CLONTECH Lab., Palo Alto, CA, USA) has been modified to the dual-cassette expression vectors named pEGFP-Ks by the relocation of its EGFP expression cassette. We have precisely monitored the cells transfected with this vector on our custom culture dishes, thereby bypassing the need for selection agent or fluorescent cell sorting. This is a useful method to screen genes encoding potential toxic or useful proteins without performing undesirable selection agent and also can be used to monitor the transfected cells for various purposes, either the inhibition or proliferation of mammalian cells for applications in biotechnology.

Comments

This article is from Experimental and Molecular Medicine 33 (2001): 174, doi: 10.1038/emm.2001.30. Posted with permission.

Rights

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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