Campus Units

Neuroscience, Zoology, Veterinary Anatomy

Document Type

Article

Publication Version

Published Version

Publication Date

1997

Journal or Book Title

Pakistan Veterinary Journal

Volume

17

Issue

3

First Page

139

Last Page

145

Abstract

Determining the birthdate of neurons and glia in relation lO the time period of neurochemical 1.nd cellular differentiation are necessary for understanding early developmental events in the central nerv.>us system (CNS). Bromodeoxyuridine (BrdU). a thymidine analog has recently become an alternative to the tritiated thymidine autoradiography for the study of cytogenesis in the CNS. Our laboratory utilizes the Brazilian opossum, Monodelphis domestica to study the ontogeny of neuropeptide systems and the development of the visual system in the mammalian brain. Opossum pups are born in an extremely immature state before neurogenesis and morphogenesis are completed and provide an excellent experimental model for developmental studies. To this end, we have developed single, double. and triple label BrctU immunohistochemical procedures for simultaneous study of cytogenesis and cellular differentiation in the opossum brain and retina. These methods are simple, rapid, and very effective and could be widely applied for understanding developmental events in the CNS.

Comments

This article is from Pakistan Veterinary Journal 17 (1997): 139. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

Copyright Owner

Pakistan Veterinary Journal

Language

en

File Format

application/pdf

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