Agricultural and Biosystems Engineering Publications
Campus Units
Agricultural and Biosystems Engineering, Bioinformatics and Computational Biology
Document Type
Article
Publication Version
Published Version
Publication Date
9-17-2019
Journal or Book Title
PLoS ONE
Volume
14
Issue
9
First Page
e0222470
Research Focus Area(s)
Land and Water Resources Engineering
DOI
10.1371/journal.pone.0222470
Abstract
Manure from animals that have been treated with antibiotics is often used to fertilize agricultural soils and its application has previously been shown to enrich for genes associated with antibiotic resistance in agroecosystems. To investigate the magnitude of this effect, we designed a column experiment simulating manure-treated agricultural soil that utilizes artificial subsurface drainage to determine the duration and extent which this type of manure fertilization impacts the set of genes associated with antibiotic resistance in drainage water. We classified ARGs in manure-treated drainage effluent water by its source of origin. Overall, we found that 61% and 7% of the total abundance of ARGs found in drainage water samples could be attributed to manure enrichment and manure addition, respectively. Among these ARGs, we identified 75 genes unique to manure that persisted in both soil and drainage water throughout a drainage season typical of the Upper Midwestern United States. While most of these genes gradually decreased in abundance over time, the IS6100-associated tet(33) gene accrued. These results demonstrate the influence of manure applications on the composition of the resistome observed in agricultural drainage water and highlight the importance of anthropogenic ARGs in the environment.
Access
Open
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Smith, Schuyler D.; Colgan, Phillip; Yang, Fan; Rieke, Elizabeth L.; Soupir, Michelle L.; Moorman, Thomas B.; Allen, Heather K.; and Howe, Adina, "Investigating the dispersal of antibiotic resistance associated genes from manure application to soil and drainage waters in simulated agricultural farmland systems" (2019). Agricultural and Biosystems Engineering Publications. 1074.
https://lib.dr.iastate.edu/abe_eng_pubs/1074
Included in
Bioinformatics Commons, Bioresource and Agricultural Engineering Commons, Computational Biology Commons, Environmental Indicators and Impact Assessment Commons, Genomics Commons
Comments
This article is published as Smith, Schuyler D., Phillip Colgan, Fan Yang, Elizabeth L. Rieke, Michelle L. Soupir, Thomas B. Moorman, Heather K. Allen, and Adina Howe. "Investigating the dispersal of antibiotic resistance associated genes from manure application to soil and drainage waters in simulated agricultural farmland systems." PLoS ONE 14, no. 9 (2019): e0222470. DOI: 10.1371/journal.pone.0222470. Posted with permission.