Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Bioinformatics and Computational Biology

Document Type

Article

Publication Version

Published Version

Publication Date

9-17-2019

Journal or Book Title

PLoS ONE

Volume

14

Issue

9

First Page

e0222470

Research Focus Area(s)

Land and Water Resources Engineering

DOI

10.1371/journal.pone.0222470

Abstract

Manure from animals that have been treated with antibiotics is often used to fertilize agricultural soils and its application has previously been shown to enrich for genes associated with antibiotic resistance in agroecosystems. To investigate the magnitude of this effect, we designed a column experiment simulating manure-treated agricultural soil that utilizes artificial subsurface drainage to determine the duration and extent which this type of manure fertilization impacts the set of genes associated with antibiotic resistance in drainage water. We classified ARGs in manure-treated drainage effluent water by its source of origin. Overall, we found that 61% and 7% of the total abundance of ARGs found in drainage water samples could be attributed to manure enrichment and manure addition, respectively. Among these ARGs, we identified 75 genes unique to manure that persisted in both soil and drainage water throughout a drainage season typical of the Upper Midwestern United States. While most of these genes gradually decreased in abundance over time, the IS6100-associated tet(33) gene accrued. These results demonstrate the influence of manure applications on the composition of the resistome observed in agricultural drainage water and highlight the importance of anthropogenic ARGs in the environment.

Comments

This article is published as Smith, Schuyler D., Phillip Colgan, Fan Yang, Elizabeth L. Rieke, Michelle L. Soupir, Thomas B. Moorman, Heather K. Allen, and Adina Howe. "Investigating the dispersal of antibiotic resistance associated genes from manure application to soil and drainage waters in simulated agricultural farmland systems." PLoS ONE 14, no. 9 (2019): e0222470. DOI: 10.1371/journal.pone.0222470. Posted with permission.

Access

Open

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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