Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Environmental Science, Chemical and Biological Engineering, Veterinary Microbiology and Preventive Medicine, Microbiology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

7-8-2020

Journal or Book Title

Nanoscale

Research Focus Area(s)

Biological and Process Engineering and Technology, Animal Production Systems Engineering

DOI

10.1039/D0NR03680C

Abstract

Compared with subcutaneous or intramuscular routes for vaccination, vaccine delivery via gastrointestinal mucosa has tremendous potential as it is easy to administer and pain free. Robust immune responses can be triggered successfully once vaccine carried antigen reaches the mucosal associated lymphoid sites (e.g., Peyer’s patches). However, the absence of an efficient delivery method has always been an issue for successful oral vaccine development. In our study, inspired by mammalian orthoreovirus (MRV) transport into gut mucosal lymphoid tissue via Microfold cells (M cells), artificial virus-like nanocarriers (AVN), consisting of gold nanocages functionalized with the 1 protein from mammalian reovirus (MRV), were tested as an effective oral vaccine delivery vehicle targeting M cells. AVN was shown to have a significantly higher transport compared to other experimental groups across mouse organoid monolayers containing M cells. These findings suggest that AVN has the potential to be an M cell-specific oral vaccine/drug delivery vehicle.

Comments

This is a manuscript of an article published as Tong, Tianjian, Yijun Qi, Luke Bussiere, Michael J. Wannemuehler, Cathy L. Miller, Chenxu Yu, and Qun Wang. "Transport of Artificial Virus-like Nanocarriers (AVN) through intestinal monolayer via Microfold cells." Nanoscale (2020). DOI: 10.1039/D0NR03680C. Posted with permission.

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Open

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

Copyright Owner

The Author(s)

Language

en

File Format

application/pdf

Published Version

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