Agricultural and Biosystems Engineering, Chemistry, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Veterinary Diagnostic and Production Animal Medicine, Veterinary Microbiology and Preventive Medicine, Environmental Science, Toxicology
Journal or Book Title
Frontiers in Bioengineering and Biotechnology
Research Focus Area(s)
Biological and Process Engineering and Technology
Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, and (4) verify that the collection system (impingers) performed similarly. The system for virus aerosolization was built and tested (Obj. 1). The uniform airflow distribution was confirmed using a physical tracer (p-value = 0.148, 0.357, 0.846) (Obj. 4). These results showed that the readiness of the system for research on virus aerosolization and treatment (e.g., by ultraviolet light), as well as its potential use for research on other types of airborne pathogens and their mitigation on a laboratory scale.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Li, Koziel, Zimmerman, Hoff, Zhang, Cheng, Yim-Im, Lee, Chen, and Jenks
Li, Peiyang; Koziel, Jacek A.; Zimmerman, Jeffrey J.; Hoff, Steven J.; Zhang, Jianqiang; Cheng, Ting-Yu; Yim-Im, Wannarat; Lee, Myeongseong; Chen, Baitong; and Jenks, William S., "Designing and Testing of a System for Aerosolization and Recovery of Viable Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Theoretical and Engineering Considerations" (2021). Agricultural and Biosystems Engineering Publications. 1183.