Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Chemistry, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Veterinary Diagnostic and Production Animal Medicine, Veterinary Microbiology and Preventive Medicine, Environmental Science, Toxicology

Document Type


Publication Version

Published Version

Publication Date


Journal or Book Title

Frontiers in Bioengineering and Biotechnology



First Page


Research Focus Area(s)

Biological and Process Engineering and Technology




Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, and (4) verify that the collection system (impingers) performed similarly. The system for virus aerosolization was built and tested (Obj. 1). The uniform airflow distribution was confirmed using a physical tracer (p-value = 0.148, 0.357, 0.846) (Obj. 4). These results showed that the readiness of the system for research on virus aerosolization and treatment (e.g., by ultraviolet light), as well as its potential use for research on other types of airborne pathogens and their mitigation on a laboratory scale.


This article is published as Li, Peiyang, Jacek Koziel, Jeffrey Zimmerman, Steven Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, and William Jenks. "Designing and Testing of a System for Aerosolization and Recovery of Viable Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Theoretical and Engineering Considerations." Frontiers in Bioengineering and Biotechnology 9 (2021): 659609. DOI: 10.3389/fbioe.2021.659609. Posted with permission.



Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright Owner

Li, Koziel, Zimmerman, Hoff, Zhang, Cheng, Yim-Im, Lee, Chen, and Jenks



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