Agricultural and Biosystems Engineering Publications
Campus Units
Agricultural and Biosystems Engineering, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Environmental Science, Toxicology, Chemistry, Veterinary Diagnostic and Production Animal Medicine, Veterinary Microbiology and Preventive Medicine
Document Type
Article
Publication Version
Submitted Manuscript
Publication Date
2-10-2021
Journal or Book Title
Preprints
First Page
2021020253
Research Focus Area(s)
Biological and Process Engineering and Technology
DOI
10.20944/preprints202102.0253.v1
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, (4) verify that the collection system (impingers) performed similarly. The system for virus aerosolization was built and tested (Obj. 1). The uniform airflow distribution was confirmed using a physical tracer (<12% relative standard deviation) for all treatments and sound engineering control of flow rates (Obj. 2). Theoretical uncertainty analyses and mass balance calculations showed <3% loss of air mass flow rate between the inlet and outlet (Obj. 3). A comparison of TCID50 values among impinger fluids showed no statistical difference between any two of the three trials (p-value = 0.148, 0.357, 0.846) (Obj. 4). These results showed that the readiness of the system for research on virus aerosolization and treatment (e.g., by ultraviolet light), as well as its potential use for research on other types of airborne pathogens and their mitigation on a laboratory scale.
Access
Open
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Author(s)
Copyright Date
2021
Language
en
File Format
application/pdf
Recommended Citation
Li, Peiyang; Koziel, Jacek A.; Zimmerman, Jeffrey J.; Hoff, Steven J.; Zhang, Jianqiang; Cheng, Ting-Yu; Yim-Im, Wannarat; Lee, Myeongseong; Chen, Baitong; and Jenks, William S., "Designing and Testing of a System for Aerosolization and Recovery of Viable Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Theoretical and Engineering Considerations" (2021). Agricultural and Biosystems Engineering Publications. 1183.
https://lib.dr.iastate.edu/abe_eng_pubs/1183
Included in
Bioresource and Agricultural Engineering Commons, Environmental Chemistry Commons, Veterinary Infectious Diseases Commons
Comments
This is a pre-print of the article Li, Peiyang, Jacek Koziel, Jeffrey Zimmerman, Steven Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, and William Jenks. "Designing and Testing of a System for Aerosolization and Recovery of Viable Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Theoretical and Engineering Considerations." (2021): 2021020253. DOI: 10.20944/preprints202102.0253.v1. Posted with permission.