Agricultural and Biosystems Engineering Publications
Campus Units
Agricultural and Biosystems Engineering, Food Science and Human Nutrition
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
4-30-2021
Journal or Book Title
Toxicon
Volume
194
First Page
17
Last Page
22
Research Focus Area(s)
Biological and Process Engineering and Technology
DOI
10.1016/j.toxicon.2021.02.008
Abstract
Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and A. parasiticus, and is a known carcinogen in humans and animals. High voltage atmospheric cold plasma (HVACP) technology has already shown promise to decontaminate AFB1 in food and feed. This study aimed to investigate the cytotoxicity of AFB1 after HVACP treatment. AFB1 (100 μM) was treated at 85 kV with HVACP for 0, 2, 5, 10, and 20 min. HepG2 cells were exposed to HVACP-treated AFB1 for 72 h and assessed for cell viability, caspase-3 activity, DNA fragmentation, and protein carbonyls for each treatment time. Cell viability, caspase-3 activity, DNA fragmentation levels, and protein carbonyls contents of HepG2 cells exposed to HVACP-treated AFB1 after 20 min was not significantly different compared to non-exposed HepG2 cells (P > 0.05). However, their contents were significantly higher in non-exposed cells compared to the other HVACP treatment times (P < 0.01). Twenty minutes of HVACP treatment for AFB1 significantly reduced AFB1 cytotoxicity and oxidative damage and showed potential as a safe aflatoxin decontamination technology.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Copyright Owner
Elsevier Ltd.
Copyright Date
2021
Language
en
File Format
application/pdf
Recommended Citation
Nishimwe, Kizito; Agbemafle, Isaac; Reddy, Manju B.; Keener, Kevin; and Maier, Dirk E., "Cytotoxicity assessment of Aflatoxin B1 after high voltage atmospheric cold plasma treatment" (2021). Agricultural and Biosystems Engineering Publications. 1187.
https://lib.dr.iastate.edu/abe_eng_pubs/1187
Included in
Agriculture Commons, Bioresource and Agricultural Engineering Commons, Food Science Commons
Comments
This is a manuscript of an article published as Nishimwe, Kizito, Isaac Agbemafle, Manju B. Reddy, Kevin Keener, and Dirk E. Maier. "Cytotoxicity Assessment of Aflatoxin B1 after High Voltage Atmospheric Cold Plasma Treatment." 194 Toxicon (2021): 17. DOI: 10.1016/j.toxicon.2021.02.008. Posted with permission.