Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Food Science and Human Nutrition

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

4-30-2021

Journal or Book Title

Toxicon

Volume

194

First Page

17

Last Page

22

Research Focus Area(s)

Biological and Process Engineering and Technology

DOI

10.1016/j.toxicon.2021.02.008

Abstract

Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and A. parasiticus, and is a known carcinogen in humans and animals. High voltage atmospheric cold plasma (HVACP) technology has already shown promise to decontaminate AFB1 in food and feed. This study aimed to investigate the cytotoxicity of AFB1 after HVACP treatment. AFB1 (100 μM) was treated at 85 kV with HVACP for 0, 2, 5, 10, and 20 min. HepG2 cells were exposed to HVACP-treated AFB1 for 72 h and assessed for cell viability, caspase-3 activity, DNA fragmentation, and protein carbonyls for each treatment time. Cell viability, caspase-3 activity, DNA fragmentation levels, and protein carbonyls contents of HepG2 cells exposed to HVACP-treated AFB1 after 20 min was not significantly different compared to non-exposed HepG2 cells (P > 0.05). However, their contents were significantly higher in non-exposed cells compared to the other HVACP treatment times (P < 0.01). Twenty minutes of HVACP treatment for AFB1 significantly reduced AFB1 cytotoxicity and oxidative damage and showed potential as a safe aflatoxin decontamination technology.

Comments

This is a manuscript of an article published as Nishimwe, Kizito, Isaac Agbemafle, Manju B. Reddy, Kevin Keener, and Dirk E. Maier. "Cytotoxicity Assessment of Aflatoxin B1 after High Voltage Atmospheric Cold Plasma Treatment." 194 Toxicon (2021): 17. DOI: 10.1016/j.toxicon.2021.02.008. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

Elsevier Ltd.

Language

en

File Format

application/pdf

Available for download on Saturday, February 19, 2022

Published Version

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