Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Egg Industry Center, Bioinformatics and Computational Biology

Document Type

Article

Publication Version

Published Version

Publication Date

8-3-2021

Journal or Book Title

PLoS ONE

Volume

16

Issue

8

First Page

e0254730

Research Focus Area(s)

Occupational Safety Engineering

DOI

10.1371/journal.pone.0254730

Abstract

Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit’s microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities’ role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.

Comments

This article is published as Yang, Fan, Daniel S. Andersen, Steven Trabue, Angela D. Kent, Laura M. Pepple, Richard S. Gates, and Adina S. Howe. "Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages." PLoS ONE 16, no. 8 (2021): e0254730. DOI: 10.1371/journal.pone.0254730.

Access

Open

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

Share

COinS