The use of biotechnology in food and agricultural applications has increased greatly during the past decade and is considered by many to be a controversial topic. Drawing upon a previous national study, a new survey was conducted of U.S. and international college students at a large, land-grant, Research University to determine factors that may affect opinions about genetically modified (GM) food products. Factors examined included nationality, discipline area of study, perceptions of safety, and awareness and levels of acceptance regarding GM food. Results indicated students born outside the United States had more negative opinions about genetically modified foods than did American-born students. Students who were studying a physical science-based curriculum had a more positive opinion of GM food than did students studying a curriculum that was not based in the physical science. In addition, students who reported a higher level of acceptance of genetically modified foods felt more positively about the safety of the technology.