
Agricultural and Biosystems Engineering Publications
Document Type
Article
Publication Version
Published Version
Publication Date
5-26-2015
Journal or Book Title
Chromatography
Volume
2
Issue
2
First Page
265
Last Page
276
DOI
10.3390/chromatography2020265
Abstract
Insects trigger plants to release volatile compounds that mediate the interaction with both pest and beneficial insects. Soybean aphids (Aphis glycines) induces soybean (Glycine max) leaves to produce volatiles that attract predators of the aphid. In this research, we describe the use of solid-phase microextraction (SPME) for extraction of volatiles from A. glycines-infested plant. Objectives were to (1) determine if SPME can be used to collect soybean plant volatiles and to (2) use headspace SPME-GC-MS approach to screen compounds associated with A. glycines-infested soybeans, grown in the laboratory and in the field, to identify previously known and potentially novel chemical markers of infestation. A total of 62 plant volatiles were identified, representing 10 chemical classes. 39 compounds had not been found in previous studies of soybean volatile emissions. 3-hexen-1-ol, dimethyl nonatriene, indole, caryophyllene, benzaldehyde, linalool, methyl salicylate (MeSA), benzene ethanol, and farnesene were considered herbivore-induced plant volatiles (HIPVs). For reproductive field-grown soybeans, three compounds were emitted in greater abundance from leaves infested with A. glycines, cis-3-hexen-1-ol acetate, MeSA and farnesene. In summary, SPME can detect the emission of HIPVs from plants infested with insect herbivores.
Access
Open
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The authors
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Cai, Lingshuang; Koziel, Jacek A.; and O'Neal, Matthew E., "Studying Plant–Insect Interactions with Solid Phase Microextraction: Screening for Airborne Volatile Emissions Response of Soybeans to the Soybean Aphid, Aphis glycines Matsumura (Hemiptera: Aphididae)" (2015). Agricultural and Biosystems Engineering Publications. 709.
https://lib.dr.iastate.edu/abe_eng_pubs/709
Included in
Agriculture Commons, Bioresource and Agricultural Engineering Commons, Entomology Commons
Comments
This article is from Chromatography 2015, 2(2), 265-276; doi:10.3390/chromatography2020265. Posted with permission.