Agricultural and Biosystems Engineering Publications

Document Type

Article

Publication Version

Published Version

Publication Date

5-26-2015

Journal or Book Title

Chromatography

Volume

2

Issue

2

First Page

265

Last Page

276

DOI

10.3390/chromatography2020265

Abstract

Insects trigger plants to release volatile compounds that mediate the interaction with both pest and beneficial insects. Soybean aphids (Aphis glycines) induces soybean (Glycine max) leaves to produce volatiles that attract predators of the aphid. In this research, we describe the use of solid-phase microextraction (SPME) for extraction of volatiles from A. glycines-infested plant. Objectives were to (1) determine if SPME can be used to collect soybean plant volatiles and to (2) use headspace SPME-GC-MS approach to screen compounds associated with A. glycines-infested soybeans, grown in the laboratory and in the field, to identify previously known and potentially novel chemical markers of infestation. A total of 62 plant volatiles were identified, representing 10 chemical classes. 39 compounds had not been found in previous studies of soybean volatile emissions. 3-hexen-1-ol, dimethyl nonatriene, indole, caryophyllene, benzaldehyde, linalool, methyl salicylate (MeSA), benzene ethanol, and farnesene were considered herbivore-induced plant volatiles (HIPVs). For reproductive field-grown soybeans, three compounds were emitted in greater abundance from leaves infested with A. glycines, cis-3-hexen-1-ol acetate, MeSA and farnesene. In summary, SPME can detect the emission of HIPVs from plants infested with insect herbivores.

Comments

This article is from Chromatography 2015, 2(2), 265-276; doi:10.3390/chromatography2020265. Posted with permission.

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Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The authors

Language

en

File Format

application/pdf

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