Agricultural and Biosystems Engineering Publications
Campus Units
Agricultural and Biosystems Engineering
Document Type
Article
Publication Version
Published Version
Publication Date
2015
Journal or Book Title
The ISME Journal
DOI
10.1038/ismej.2015.183
Abstract
To improve our understanding of the stability of mammalian intestinal communities, we characterized the responses of both bacterial and viral communities in murine fecal samples to dietary changes between high- and low-fat (LF) diets. Targeted DNA extraction methods for bacteria, virus-like particles and induced prophages were used to generate bacterial and viral metagenomes as well as 16S ribosomal RNA amplicons. Gut microbiome communities from two cohorts of C57BL/6 mice were characterized in a 6-week diet perturbation study in response to high fiber, LF and high-refined sugar, milkfat (MF) diets. The resulting metagenomes from induced bacterial prophages and extracellular viruses showed significant overlap, supporting a largely temperate viral lifestyle within these gut microbiomes. The resistance of baseline communities to dietary disturbances was evaluated, and we observed contrasting responses of baseline LF and MF bacterial and viral communities. In contrast to baseline LF viral communities and bacterial communities in both diet treatments, baseline MF viral communities were sensitive to dietary disturbances as reflected in their non-recovery during the washout period. The contrasting responses of bacterial and viral communities suggest that these communities can respond to perturbations independently of each other and highlight the potentially unique role of viruses in gut health.
Access
Open
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
International Society for Microbial Ecology
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Howe, Adina C.; Ringus, Daina L.; Williams, Ryan J.; Choo, Zi-Ning; Greenwald, Stephanie M.; Owens, Sarah M.; Coleman, Maureen L.; Meyer, Folker; and Chang, Eugene B., "Divergent responses of viral and bacterial communities in the gut microbiome to dietary disturbances in mice" (2015). Agricultural and Biosystems Engineering Publications. 735.
https://lib.dr.iastate.edu/abe_eng_pubs/735
Included in
Agriculture Commons, Bioresource and Agricultural Engineering Commons, Environmental Microbiology and Microbial Ecology Commons, Veterinary Microbiology and Immunobiology Commons
Comments
This article is from The ISME Journal (2015), doi:10.1038/ismej.2015.183. Posted with permission.