Agricultural and Biosystems Engineering Publications

Campus Units

Aerospace Engineering, Agricultural and Biosystems Engineering, Food Science and Human Nutrition, Egg Industry Center

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Publication Version

Published Version

Publication Date


Journal or Book Title

Annals of Agricultural and Environmental Medicine





First Page


Last Page


Research Focus Area(s)

Animal Production Systems Engineering




Spray-application of membraneless acidic electrolyzed water (MLAEW) is a novel technique for disinfection in livestock houses. This study investigated the loss of free chlorine (FC – the major germicidal component in MLAEW) over distance during spraying, as affected by air temperature and initial FC concentration. The anti-microbial effect of MLAEW on airborne bacteria from an aviary laying-hen house was examined. materials and methods. MLAEW was prepared at two FC concentrations: app. 15 and 60 mg L -1 , and sprayed at three air temperatures (18, 25, 32 °C). The original MLAEW solution and MLAEW aerosols collected at 0, 25, and 50 cm from the spray nozzle were analyzed for FC concentrations. Bacteria were immersed into these MLAEW samples and numerated for viable count after 0.5, 2 and 5-min treatments. results. MLAEW aerosols collected at 0 cm lost 11.7–13.2% FC, compared with the original MLAEW solution. This initial loss was affected neither by the initial FC concentration (P = 0.13) nor by air temperature (P = 0.57). The rate of FC loss during travelling was 0.79–0.87 % per cm of aerosol travel distance (% cm -1 ) at 18 °C, 1.08–1.15 % cm -1 at 25 °C, and 1.35–1.49% cm -1 at 32 °C. This travelling loss was affected by air temperature (P = 0.02), but not by initial FC concentration (P = 0.38). Bacteria were completely inactivated at 0.5 min when treated with MLAEW samples with FC > 16.8 mg L -1 , in 2 min when FC > 13.8 mg L -1 , and in 5 min when FC > 7.2 mg L -1 . conclusion. Airborne bacteria from aviary hen house can be effectively inactivated by MLAEW with adequate FC concentration and contact time. During spraying, the anti-microbial efficacy of MLAEW aerosols decreased over distance due to FC loss which exacerbated at higher air temperatures.


This article is published as Zhao, Y, H. Xin, D. Zhao, W. Zheng, W. Tian, H. Ma, K. Liu, H. Hu, T. Wang, M.L. Soupir. 2014. Free chlorine loss during spray of membrane-less acidic electrolyzed water and its antimicrobial effect on airborne bacteria from poultry house. Annals of Agricultural and Environmental Medicine 21(2): 249-255. DOI: 10.5604/1232-1966.1108585

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Creative Commons Attribution-NonCommercial 4.0 International License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

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Institute of Rural Health



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