Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

1-15-2018

Journal or Book Title

FEMS Microbiology Ecology

Research Focus Area(s)

Biological and Process Engineering and Technology

DOI

10.1093/femsec/fiy006

Abstract

Use of antibiotics in human and animal medicine has applied selective pressure for the global dissemination of antibiotic-resistant bacteria. Therefore, it is of interest to develop strategies to mitigate the continued amplification and transmission of resistance genes in environmental reservoirs such as farms, hospitals, and watersheds. However, the efficacy of mitigation strategies is difficult to evaluate because it is unclear which resistance genes are important to monitor, and which primers to use to detect those genes. Here we evaluated the diversity of one type of macrolide antibiotic resistance gene (erm) in one type of environment (manure) to determine which primers would be most informative to use in a mitigation study of that environment. We analyzed all known erm genes and assessed the ability of previously published erm primers to detect the diversity. The results showed that all known erm resistance genes group into 66 clusters, and 25 of these clusters (40%) can be targeted with primers found in the literature. These primers can target 74–85% of the ermgene diversity in the manures analyzed.

Comments

This is a manuscript of an article published as Choi, Jinlyung, Elizabeth L. Rieke, Thomas B. Moorman, Michelle L. Soupir, Heather K. Allen, Schuyler D. Smith, and Adina Howe. "Practical implications of erythromycin resistance gene diversity on surveillance and monitoring of resistance." FEMS Microbiology Ecology (2018). doi: 10.1093/femsec/fiy006. Posted with permission.

Access

Open

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

FEMS

Language

en

File Format

application/pdf

Published Version

Share

COinS