Agricultural and Biosystems Engineering Publications
Campus Units
Agricultural and Biosystems Engineering, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Toxicology
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
8-2017
Journal or Book Title
Chromatographia
Volume
80
Issue
8
First Page
1249
Last Page
1256
Research Focus Area(s)
Biological and Process Engineering and Technology
DOI
10.1007/s10337-017-3340-0
Abstract
There is a great demand for simple, fast and accurate methods for quantification of volatile organic contaminants in soil samples. Solid-phase microextraction (SPME) has a huge potential for this purpose, but its application is limited by insufficient accuracy caused by a matrix effect. The aim of this research was to develop the method for BTEX quantification in soil using combined standard addition (SA) and internal standard (IS) calibration. Deuterated benzene (benzene-d6) was used as the internal standard for all analytes. The optimized method includes spiking replicate samples with different concentrations of BTEX standards and the same concentration of benzene-d6, equilibration of soil samples at 40 °C during 2 h, and SPME–GC–MS analysis. Precision and accuracy of IS and SA methods were compared on different soil matrices. Combined SA + IS method provided more precise calibration plots compared to the conventional SA calibration. The SA + IS calibration provided more precise and accurate results compared with a reference method based on solvent extraction followed by GC–MS when applied to BTEX quantification in real soil samples (spiked with diesel fuel and aged). Recoveries of BTEX from soil samples spiked with known concentrations of analytes using the developed method were in the range of 73–130% with RSD values less than 15% for all BTEX. The proposed simultaneous standard addition and internal standard approach can be advantageous and adopted for improved quantification of other toxic VOCs in soil.
Copyright Owner
Springer-Verlag GmbH Germany
Copyright Date
2017
Language
en
File Format
application/pdf
Recommended Citation
Orazbayeva, Dina; Kenessov, Bulat; Koziel, Jacek A.; Nassyrova, Dayana; and Lyabukhova, Nadezhda V., "Quantification of BTEX in Soil by Headspace SPME–GC–MS Using Combined Standard Addition and Internal Standard Calibration" (2017). Agricultural and Biosystems Engineering Publications. 895.
https://lib.dr.iastate.edu/abe_eng_pubs/895
Included in
Agriculture Commons, Biochemical and Biomolecular Engineering Commons, Bioresource and Agricultural Engineering Commons
Comments
This is a manuscript of an article published as Orazbayeva, Dina, Bulat Kenessov, Jacek A. Koziel, Dayana Nassyrova, and Nadezhda V. Lyabukhova. "Quantification of BTEX in Soil by Headspace SPME–GC–MS Using Combined Standard Addition and Internal Standard Calibration." Chromatographia 80, no. 8 (2017): 1249-1256. The final publication is available at Springer via DOI: 10.1007/s10337-017-3340-0. Posted with permission.