Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Toxicology

Document Type


Publication Version

Accepted Manuscript

Publication Date


Journal or Book Title

Journal of Chromatography A





First Page


Last Page


Research Focus Area(s)

Biological and Process Engineering and Technology




There has been considerable public interest and a growing number of scientific studies linking certain phenolic compounds in grapes and wines, particularly trans-resveratrol (trans-3,5,4′-trihydroxystilbene, TRA), to human health benefits. Typical TRA concentrations in wine are very low. It is a polar compound with very low volatility, which makes it difficult to extract and to separate on a gas chromatography (GC) column without derivatization. In this study, a new method for trace analysis of TRA was developed using solid-phase microextraction (SPME) with on-fiber silylation derivatization. Multidimensional GC equipped with a heartcut valve and cryogenic focusing was coupled with a mass-selective detector and used for improved separations and analysis. The effects of SPME fiber selection, extraction time, temperature, and desorption time were investigated. The derivatization conditions, time/temperature and the volume of derivatization reagent were also optimized. The calibration curve was linear over the concentration range from 10 ng L−1 to 5 mg L−1, with a correlation coefficient of 0.9996. The average recovery of TRA in red wine was 83.6 ± 5.6%. The method detection limit (MDL) for TRA in ethanol:water (12.5:87.5, v/v) solution in this study was 7.08 ng L−1 whereas the MDL for TRA in pure water was 2.85 ng L−1. The new method was used to test the TRA content in six selected Iowa red wine samples. Measured concentrations varied from 12.72 to 851.9 μg L−1.


This is a manuscript of an article published as Cai, Lingshuang, Jacek A. Koziel, Murli Dharmadhikari, and J. Hans van Leeuwen. "Rapid determination of trans-resveratrol in red wine by solid-phase microextraction with on-fiber derivatization and multidimensional gas chromatography–mass spectrometry." Journal of Chromatography A 1216, no. 2 (2009): 281-287. DOI: 10.1016/j.chroma.2008.11.050. Posted with permission.

Copyright Owner

Elsevier B.V.



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Published Version