Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Toxicology

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Publication Version

Accepted Manuscript

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Journal or Book Title

Journal of Separation Science





First Page


Last Page


Research Focus Area(s)

Biological and Process Engineering and Technology




A new approach is described to capture nano‐size aerosols on internally‐cooled micro tubing of the solid‐phase microextraction (SPME) device followed by convenient introduction of the collected analytes into analytical instrument. Particles were generated using an aerosol formation by homogeneous nucleation of an organic vapor, and subsequent growth to nano‐size particles by coagulation of decanedioic acid, bis[2‐ethylhexyl] ester (DEHS). The approach was validated by using carbon dioxide‐cooled micro tubing to collect the nanosize DEHS particles followed by analyses on GC‐flame ionization detector (FID). Particle size ranged from 150 to 590 nm. Temperature difference between the SPME device and DEHS particles mixture created a temperature gradient and resulted in thermophoretic effect that was determining the extraction rate. SPME device was cooled to as low as –75°C, while the DEHS remained close to room temperature. Several aspects of nanoparticle sampling were tested to demonstrate the principle of the sampling approach. These included the effects of thermal gradient, sample flow rate, sampling time, CO2 delivery mode (constant coolant delivery vs. constant temperature), and particle size. Results were normalized to measure particulate concentrations using direct sampling with PTFE filters. Nanoparticle extractions of DEHS mass were proportional to sampling time. Normalized mass of DEHS extracted increased with increase in temperature gradient and with increase of the cross flow velocity. Preliminary results indicate that the variation of heat transfer boundary layer caused by the variation in the cross flow velocity produce self‐compensating effect at constant coolant delivery, indicating that this approach could be used for field determinations including the time‐weighted average sampling of nanoparticles. Thus, it may be possible to develop simple device based on this concept for field applications.


This is the peer-reviewed version of the following article: Koziel, Jacek A., Shokouh Hosseinzadeh Haddadi, Wolfgang Koch, and Janusz Pawliszyn. "Sampling and analysis of nanoparticles with cold fibre SPME device." Journal of Separation Science 32, no. 11 (2009): 1975-1980, which has been published in final form atDOI: 10.1002/jssc.200900023. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Posted with permission.

Copyright Owner

WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim



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Published Version