Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Civil, Construction and Environmental Engineering, Food Science and Human Nutrition, Toxicology, Animal Science, Egg Industry Center

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

11-2006

Journal or Book Title

Analytical and Bioanalytical Chemistry

Volume

386

Issue

6

First Page

1791

Last Page

1802

Research Focus Area(s)

Animal Production Systems Engineering

DOI

10.1007/s00216-006-0799-1

Abstract

Volatile organic compounds (VOCs) and odors in cattle rumen gas have been characterized by in-vivo headspace sampling by solid-phase microextraction (SPME) and analysis by gas chromatography–mass spectrometry–olfactometry (GC–MS–O). A novel device enabling headspace SPME (HS-SPME) sampling through a cannula was designed, refined, and used to collect rumen gas samples from steers. A Carboxen–polydimethylsiloxane (PDMS) fiber (85 μm) was used for SPME sampling. Fifty VOCs from ten chemical groups were identified in the rumen headspace. The VOCs identified had a wide range of molecular weight (MW) (34 to 184), boiling point (−63.3 to 292 °C), vapor pressure (1.05 × 10−5 to 1.17 × 102 Pa), and water solubility (0.66 to 1 × 106 mg L−1). Twenty-two of the compounds have a published odor detection thresholds (ODT) of less than 1 ppm. More than half of the compounds identified are reactive and have an estimated atmospheric lifetime of <24 h. The amounts of VFAs, sulfide compounds, phenolic compounds, and skatole, and the odor intensity of VFAs and sulfide compounds in the rumen gas were all higher after feeding than before feeding. These results indicate that rumen gases can be an important potential source of aerial emissions of reactive VOCs and odor. In-vivo sampling by SPME then GC–MS–O analysis can be a useful tool for qualitative characterization of rumen gases, digestion, and its relationship to odor and VOC formation.

Comments

This is a manuscript of an article published as Cai, Lingshuang, Jacek A. Koziel, Jeremiah Davis, Yin-Cheung Lo, and Hongwei Xin. "Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas, by solid-phase microextraction, and gas chromatography–mass spectrometry–olfactometry." Analytical and Bioanalytical Chemistry 386, no. 6 (2006): 1791-1802.The final publication is available at Springer via DOI: 10.1007/s00216-006-0799-1. Posted with permission.

Copyright Owner

Springer-Verlag

Language

en

File Format

application/pdf

Published Version

Share

COinS