Agricultural and Biosystems Engineering Publications

Campus Units

Agricultural and Biosystems Engineering, Ecology, Evolution and Organismal Biology

Document Type

Article

Publication Version

Published Version

Publication Date

10-2-2018

Journal or Book Title

Frontiers in Environmental Science

Volume

6

First Page

107

Research Focus Area(s)

Land and Water Resources Engineering

DOI

10.3389/fenvs.2018.00107

Abstract

Microbial mechanisms controlling cellulose degradation in soil habitats remains a critical knowledge gap in understanding and modeling terrestrial carbon-cycling. We investigated land management and soil micro-habitat influences on soil bacterial communities and distribution of cellulose-degrading enzyme genes in three bioenergy cropping systems (corn, prairie, and fertilized prairie). Within the soil, aggregates have been examined as potential micro- habitats with specific characteristics influencing resource partitioning and regulation, thus we also investigated genes associated with cellulose degradation within soil aggregate fractions from the fertilized prairie system. Soil bacterial communities and carbon-cycling gene presence varied across land management and soil microhabitats. Examination of genes specifically involved in cellulose-degradation pathways showed high levels of redundancy across the bioenergy cropping systems, but medium macroaggregates (1,000–2,000 μm) supported greater cellulose-degrading enzyme gene abundance than other aggregate fractions and whole soil. In medium aggregates, the enriched cellulose-degrading genes were most similar to genes previously observed in Actinobacteria. These findings represent gentic potential only, and our previous work on the same samples found elevated cellulase exo-enzyme activity in microaggregates. These contrasting results emphasize the importance of measuring community, functional genes, and metabolic potentials in a coordinated manner. Together, these data indicate that location within the soil matrix matters. Overall, our results indicate that soil aggregate environments are hot-spots that select for organisms with functional attributes like cellulose degradation, and future work should further explore micro-environmental factors that affect realized C-cycling processes.

Comments

This article is published as Choi, Jinlyung, Elizabeth Bach, Jaejin Lee, Jared Flater, Shane Dooley, Adina Howe, and Kirsten Hofmockel. "Spatial structuring of cellulase gene abundance and activity in soil." Frontiers in Environmental Science 6 (2018): 107. DOI: 10.3389/fenvs.2018.00107. Posted with permission.

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Open

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Choi, Bach, Lee, Flater, Dooley, Howe and Hofmockel

Language

en

File Format

application/pdf

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