Agricultural and Biosystems Engineering Publications
Campus Units
Agricultural and Biosystems Engineering, Civil, Construction and Environmental Engineering, Veterinary Diagnostic and Production Animal Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
3-12-2018
Journal or Book Title
Separations
Volume
5
Issue
1
First Page
18
Research Focus Area(s)
Animal Production Systems Engineering
DOI
10.3390/separations5010018
Abstract
Non-invasive diagnostics and finding biomarkers of disease in humans have been a very active research area. Some of the analytical technologies used for finding biomarkers of human disease are finding their use in livestock. Non-invasive sample collection from diseased cattle using breath and headspace of fecal samples have been reported. In this work, we explore the use of volatile organic compounds (VOCs) emitted from bovine nasal secretions and serum for finding biomarkers for bovine respiratory disease (BRD). One hundred nasal swabs and 100 serum samples (n = 50 for both ‘sick’ and ‘healthy’) were collected at the time of treatment for suspected BRD. Solid-phase microextraction (SPME) was used to collect headspace samples that were analyzed using gas chromatography-mass spectrometry (GC-MS). It was possible to separate sick cattle using non-invasive analyses of nasal swabs and also serum samples by analyzing and comparing volatiles emitted from each group of samples. Four volatile compounds were found to be statistically significantly different between ‘sick’ and ‘normal’ cattle nasal swabs samples. Five volatile compounds were found to be significantly different between ‘sick’ and ‘normal’ cattle serum samples, with phenol being the common marker. Future studies are warranted to improve the extraction efficiency targeting VOCs preliminarily identified in this study. These findings bring us closer to the long-term goal of real-time, animal-side detection and separation of sick cattle.
Access
Open
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Owner
The Authors
Copyright Date
2018
Language
en
File Size
application/pdf
Recommended Citation
Maurer, Devin L.; Koziel, Jacek A.; Engelken, Terry J.; Cooper, Vickie L.; and Funk, Jenna L., "Detection of Volatile Compounds Emitted from Nasal Secretions and Serum: Towards Non-Invasive Identification of Diseased Cattle Biomarkers" (2018). Agricultural and Biosystems Engineering Publications. 958.
https://lib.dr.iastate.edu/abe_eng_pubs/958
Included in
Bioresource and Agricultural Engineering Commons, Environmental Health and Protection Commons, Veterinary Infectious Diseases Commons
Comments
This article is published as Maurer, Devin L., Jacek A. Koziel, Terry J. Engelken, Vickie L. Cooper, and Jenna L. Funk. "Detection of Volatile Compounds Emitted from Nasal Secretions and Serum: Towards Non-Invasive Identification of Diseased Cattle Biomarkers." Separations 5, no. 1 (2018): 18. DOI: 10.3390/separations5010018. Posted with permission.