Campus Units
Agronomy
Document Type
Article
Publication Version
Published Version
Publication Date
2016
Journal or Book Title
Crop Science
Volume
56
Issue
5
First Page
2379
Last Page
2389
DOI
10.2135/cropsci2015.10.0633
Abstract
Methionine is a limiting amino acid in poultry diets, so methionine supplementation is typically required to meet nutritional demands. Maize (Zea mays L.) varieties with increased methionine levels have been developed using three different approaches: (i) increased levels of the methionine-rich 10-kDa zein, (ii) disruption of protein deposition using the floury-2 allele, and (iii) recurrent selection. The goal of this study was to characterize the interactions of these three mechanisms for increasing methionine to develop optimal breeding strategies for this limiting amino acid. A complete diallel mating design was used to produce all possible hybrid combinations, which were analyzed by Griffing’s experimental Method 3, Model 1. Grain samples were analyzed for methionine concentration using a microbial method. The significantly negative general combining ability (GCA) for inbred RS2 suggests it did not perform well in hybrid combination, while the significant specific combining abilities (SCAs) suggest that some specific combinations of mechanisms worked well together in this germplasm. Analysis of grain quality traits by near-infrared spectroscopy (NIRS) revealed that the high-methionine hybrid combinations had starch and oil concentrations similar to all other hybrids but had elevated protein concentrations. In some hybrids in this study, dzr1 and recurrent selection were effective mechanisms to elevate methionine in hybrid combination and did not have an associated yield penalty relative to other hybrids produced in the study, which supports their use in a high-methionine maize breeding program.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Huffman, Ryan D.; Edwards, Jode W.; Pollak, Linda M.; and Scott, M. Paul, "Interaction of Genetic Mechanisms Regulating Methionine Concentration in Maize Grain" (2016). Agronomy Publications. 139.
https://lib.dr.iastate.edu/agron_pubs/139
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons
Comments
This article is published as Huffman, Ryan D., Jode W. Edwards, Linda M. Pollak, and M. Paul Scott. "Interaction of genetic mechanisms regulating methionine concentration in maize grain." Crop Science 56, no. 5 (2016): 2379-2389. doi: 10.2135/cropsci2015.10.0633.