Campus Units

Agronomy, Center for Crops Utilization Research, Genetics

Document Type

Article

Publication Version

Published Version

Publication Date

2008

Journal or Book Title

Cereal Chemistry

Volume

85

Issue

2

First Page

196

Last Page

201

DOI

10.1094/CCHEM-85-2-0196

Abstract

The efficiency of fractionating cereal grains (e.g., dry corn milling) can be evaluated and monitored by quantifying the proportions of seed tissues in each of the recovered fractions. The quantities of individual tissues are typically estimated using indirect methods such as quantifying fiber or ash to indicate pericarp and tip cap contents, and oil to indicate germ content. More direct and reliable methods are possible with tissue-specific markers. We used two transgenic maize lines, one containing the fluorescent protein green fluorescent protein (GFP) variant S65T expressed in endosperm, and the other containing GFP expressed in germ to determine the fate of each tissue in the dry-milling fractionation process. The two lines were dry-milled to produce three fractions (bran-, endosperm-, and germ-rich fractions) and GFP fluorescence was quantified in each fraction to estimate the tissue composition. Using a simplified laboratory dry-milling procedure and our GFP-containing grain, we determined that the endosperm-rich fraction contained 4% germ tissue, the germ-rich fraction contained 28% germ, 20% endosperm, and 52% nonendosperm and nonembryo tissues, and the bran-rich fraction contained 44% endosperm, 13% germ, and 43% nonendosperm and nonembryo tissues. GFP-containing grain can be used to optimize existing fractionation methods and to develop improved processing strategies.

Comments

This article is published as Shepherd, C. T., N. Vignaux, J. M. Peterson, M. P. Scott, and L. A. Johnson. "Dry-Milling and Fractionation of Transgenic Maize Seed Tissues with Green Fluorescent Protein as a Tissue Marker." Cereal Chemistry 85, no. 2 (2008): 196-201, doi; 10.1094/CCHEM-85-2-0196.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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