Campus Units
Agronomy, Center for Crops Utilization Research, Genetics
Document Type
Article
Publication Version
Published Version
Publication Date
2008
Journal or Book Title
Cereal Chemistry
Volume
85
Issue
2
First Page
196
Last Page
201
DOI
10.1094/CCHEM-85-2-0196
Abstract
The efficiency of fractionating cereal grains (e.g., dry corn milling) can be evaluated and monitored by quantifying the proportions of seed tissues in each of the recovered fractions. The quantities of individual tissues are typically estimated using indirect methods such as quantifying fiber or ash to indicate pericarp and tip cap contents, and oil to indicate germ content. More direct and reliable methods are possible with tissue-specific markers. We used two transgenic maize lines, one containing the fluorescent protein green fluorescent protein (GFP) variant S65T expressed in endosperm, and the other containing GFP expressed in germ to determine the fate of each tissue in the dry-milling fractionation process. The two lines were dry-milled to produce three fractions (bran-, endosperm-, and germ-rich fractions) and GFP fluorescence was quantified in each fraction to estimate the tissue composition. Using a simplified laboratory dry-milling procedure and our GFP-containing grain, we determined that the endosperm-rich fraction contained 4% germ tissue, the germ-rich fraction contained 28% germ, 20% endosperm, and 52% nonendosperm and nonembryo tissues, and the bran-rich fraction contained 44% endosperm, 13% germ, and 43% nonendosperm and nonembryo tissues. GFP-containing grain can be used to optimize existing fractionation methods and to develop improved processing strategies.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Shepherd, C. T.; Vignaux, N.; Peterson, J. M.; Scott, M. P.; and Johnson, L. A., "Dry-Milling and Fractionation of Transgenic Maize Seed Tissues with Green Fluorescent Protein as a Tissue Marker" (2008). Agronomy Publications. 142.
https://lib.dr.iastate.edu/agron_pubs/142
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Genetics and Genomics Commons, Plant Breeding and Genetics Commons
Comments
This article is published as Shepherd, C. T., N. Vignaux, J. M. Peterson, M. P. Scott, and L. A. Johnson. "Dry-Milling and Fractionation of Transgenic Maize Seed Tissues with Green Fluorescent Protein as a Tissue Marker." Cereal Chemistry 85, no. 2 (2008): 196-201, doi; 10.1094/CCHEM-85-2-0196.