Campus Units
Agronomy, Plant Biology, Genetics
Document Type
Article
Publication Version
Published Version
Publication Date
2011
Journal or Book Title
Crop Science
Volume
51
Issue
6
First Page
2716
Last Page
2720
DOI
10.2135/cropsci2011.03.0168
Abstract
Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments. Our objective was to determine if predicted epistatic interactions occur between maize (Zea mays L.) transgenes carrying zein promoters and opaque2, a transcriptional regulator of zein genes. Expression of the transgenes was significantly decreased in kernels containing the opaque2 mutation. Native zein proteins in these kernels were reduced as well. Thus, transgene expression mirrored expression of the native gene that contributed the promoter of the transgene. This work demonstrates that information about native gene expression may be useful for predicting transgene expression, reducing the risk of unintended consequences of transgene expression.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Schroder, Megan N.; Floyd, Brice E.; and Scott, M. Paul, "Maize Transgenes Containing Zein Promoters are Regulated by opaque2" (2011). Agronomy Publications. 148.
https://lib.dr.iastate.edu/agron_pubs/148
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons
Comments
This article is published as Schroder, Megan N., Brice E. Floyd, and M. Paul Scott. "Maize Transgenes Containing Zein Promoters are Regulated by." Crop science 51, no. 6 (2011): 2716-2720, doi: 10.2135/cropsci2011.03.0168.