Campus Units

Agronomy, Plant Biology, Genetics

Document Type

Article

Publication Version

Published Version

Publication Date

2011

Journal or Book Title

Crop Science

Volume

51

Issue

6

First Page

2716

Last Page

2720

DOI

10.2135/cropsci2011.03.0168

Abstract

Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments. Our objective was to determine if predicted epistatic interactions occur between maize (Zea mays L.) transgenes carrying zein promoters and opaque2, a transcriptional regulator of zein genes. Expression of the transgenes was significantly decreased in kernels containing the opaque2 mutation. Native zein proteins in these kernels were reduced as well. Thus, transgene expression mirrored expression of the native gene that contributed the promoter of the transgene. This work demonstrates that information about native gene expression may be useful for predicting transgene expression, reducing the risk of unintended consequences of transgene expression.

Comments

This article is published as Schroder, Megan N., Brice E. Floyd, and M. Paul Scott. "Maize Transgenes Containing Zein Promoters are Regulated by." Crop science 51, no. 6 (2011): 2716-2720, doi: 10.2135/cropsci2011.03.0168.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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