Campus Units

Agronomy

Document Type

Book Chapter

Publication Version

Published Version

Publication Date

2009

Journal or Book Title

Methods in Molecular Biology: Transgenic Maize

Volume

526

First Page

123

Last Page

128

DOI

10.1007/978-1-59745-494-0_10

Abstract

This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.

Comments

This chapter is from Scott, M. Paul. "Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds." Transgenic Maize: Methods and Protocols (2009): 123-128, doi: 10.1007/978-1-59745-494-0_10.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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