Journal or Book Title
Methods in Molecular Biology: Transgenic Maize
This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.
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Scott, M. Paul, "Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds" (2009). Agronomy Publications. 179.