Campus Units

Agronomy

Document Type

Book Chapter

Publication Version

Published Version

Publication Date

2009

Journal or Book Title

Methods in Molecular Biology: Transgenic Maize

Volume

526

First Page

129

Last Page

134

DOI

10.1007/978-1-59745-494-0_11

Abstract

Efficient methods to characterize transgenic plants are important to quickly understand the state of the transformant. Determining transgene copy number is an important step in transformant characterization and can differentiate between complex and simple transformation events. This knowledge can be extremely useful when determining what future experiments and uses the transgenic lines can be utilized for. The method described here uses real-time quantitative PCR to determine the transgene copy number present in the genome of the transformant. Specifically, this method measures the relative transgene copy number by comparing it with an endogenous gene with a known copy number. This method is a quick alternative to the Southern blot, a method that is commonly used to determine gene copy number, and is effective when screening large numbers of transformants.

Comments

This is a chapter from Shepherd, Colin T., Adrienne N. Moran Lauter, and M. Paul Scott. "Determination of transgene copy number by real-time quantitative PCR." Transgenic Maize: Methods and Protocols (2009): 129-134, doi: 10.1007/978-1-59745-494-0_11.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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