Campus Units

Agronomy

Document Type

Article

Publication Version

Published Version

Publication Date

1989

Journal or Book Title

Crop Science

Volume

29

Issue

4

First Page

1067

Last Page

1071

DOI

10.2135/cropsci1989.0011183X002900040050x

Abstract

Restriction fragment length polymorphisms (RFLPs) have been suggested as molecular markers to facilitate improvement of agronomic traits in maize (Zea mays L.). The objective of this study was to evaluate the utility of RFLP data in elucidating heterotic patterns among maize lines. Eight maize inbred lines and their 28 singlecross hybrids werevaluated for grain yield at two Iowa locations in each of 2 yr in a randomized-complete block design. The diallel mating design permitted estimation of general and specific combining ability effects. Restriction fragment length polymorphism analysis of inbred lines included five restriction enzymes and five eDNA and 28 genomic clones distributed over the maize genome. Restriction fragment length polymorphism patterns of crosses were predicted from analysis of the inbred parents. Genetic distances between inbred lines were estimated as modified Rogers' distance (MRD). Grain yield and specific combining ability were significantly correlated with MRD for six of the 10 chromosomes. Dispersion of inbred lines and hybrids for RFLP allele frequencies was generally consistent with expectations based on known pedigrees. Results from this study suggest RFLP analysis as a potential alternative to field testing when attempting to assign maize inbred lines to heterotic groups.

Comments

This article is published as Lee, M., E. B. Godshalk, Kr R. Lamkey, and W. W. Woodman. "Association of restriction fragment length polymorphisms among maize inbreds with agronomic performance of their crosses." Crop Science 29, no. 4 (1989): 1067-1071. doi: 10.2135/cropsci1989.0011183X002900040050x. Posted with permission.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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