Campus Units

Agronomy

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

7-1-2017

Journal or Book Title

Theoretical and Applied Genetics

Volume

130

Issue

7

First Page

1349

Last Page

1359

DOI

10.1007/s00122-017-2892-6

Abstract

Doubled haploid (DH) technology enables rapid development of homozygous lines in maize breeding programs. However, haploid genome doubling is a bottleneck for the commercialization of DH technology and is limited by haploid male fertility (HMF). This is the first study reporting the quantitative trait locus (QTL) analysis of HMF in maize. Four QTL, qhmf1, qhmf2, qhmf3, and qhmf4, controlling HMF have been identified by segregation distortion (SD) loci detection in the selected haploid population derived from ‘Yu87-1/Zheng58’. Three loci, qhmf1, qhmf2, and qhmf4, were also detected in the selected haploid population derived from ‘4F1/Zheng58’. The QTL qhmf4 showed the strongest SD in both haploid populations. Based on the sequence information of ‘Yu87-1’ and ‘Zheng58’, thirteen markers being polymorphic between the two lines were developed to saturate the qhmf4 region. A total of 8168 H1BC2 (haploid backcross generation) plants produced from ‘Yu87-1’ and ‘Zheng58’ were screened for recombinants. All the 48 recombinants were backcrossed to ‘Zheng58’ to develop H1BC3 progeny. The heterozygous H1BC3 individuals were crossed with CAU5 to induce haploids. In each H1BC3 progeny, haploids were genotyped and evaluated for anther emergence score (AES). Significant (or no significant) difference (P < 0.05) between haploids with or without ‘Yu87-1’ donor segment indicated presence or absence of qhmf4 in the donor segment. The analysis of the 48 recombinants narrowed the qhmf4 locus down to an ~800 kb interval flanked by markers IND166 and IND1668.

Comments

This is an accepted manuscript published as Ren, Jiaojiao, Penghao Wu, Xiaolong Tian, Thomas Lübberstedt, and Shaojiang Chen. "QTL mapping for haploid male fertility by a segregation distortion method and fine mapping of a key QTL qhmf4 in maize." Theoretical and Applied Genetics (2017): 1-11. doi: 10.1007/s00122-017-2892-6. Posted with permission.

Copyright Owner

Springer Verlag

Language

en

File Format

application/pdf

2017-Lubberstedt-QTL Mapping Supplement.pdf (337 kB)
Supplemental Materials

Available for download on Sunday, July 01, 2018

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