Campus Units
Agronomy, Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
2017
Journal or Book Title
Scientific Reports
Volume
7
Issue
1
First Page
3554
DOI
10.1038/s41598-017-03695-9
Abstract
Genome-wide association (GWAS) and epistatic (GWES) studies along with expression studies in soybean [Glycine max (L.) Merr.] were leveraged to dissect the genetics of Sclerotinia stem rot (SSR) [caused by Sclerotinia sclerotiorum (Lib.) de Bary], a significant fungal disease causing yield and quality losses. A large association panel of 466 diverse plant introduction accessions were phenotyped in multiple field and controlled environments to: (1) discover sources of resistance, (2) identify SNPs associated with resistance, and (3) determine putative candidate genes to elucidate the mode of resistance. We report 58 significant main effect loci and 24 significant epistatic interactions associated with SSR resistance, with candidate genes involved in a wide range of processes including cell wall structure, hormone signaling, and sugar allocation related to plant immunity, revealing the complex nature of SSR resistance. Putative candidate genes [for example, PHYTOALEXIN DEFFICIENT 4 (PAD4), ETHYLENE-INSENSITIVE 3-LIKE 1 (EIL3), and ETHYLENE RESPONSE FACTOR 1 (ERF1)] clustered into salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) pathways suggest the involvement of a complex hormonal network typically activated by both necrotrophic (ET/JA) and biotrophic (SA) pathogens supporting that S. sclerotiorum is a hemibiotrophic plant pathogen.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Owner
The Authors
Copyright Date
2017
Language
en
File Format
application/pdf
Recommended Citation
Moellers, Tara C.; Singh, Arti; Zhang, Jiaoping; Brungardt, Jae; Kabbage, Mehdi; Mueller, Daren S.; Grau, Craig R.; Ranjan, Ashish; Smith, Damon L.; Chowdy-Reddy, R. V.; and Singh, Asheesh K., "Main and epistatic loci studies in soybean for Sclerotinia sclerotiorum resistance reveal multiple modes of resistance in multi-environments" (2017). Agronomy Publications. 338.
https://lib.dr.iastate.edu/agron_pubs/338
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as Moellers, Tara C., Arti Singh, Jiaoping Zhang, Jae Brungardt, Mehdi Kabbage, Daren S. Mueller, Craig R. Grau et al. "Main and epistatic loci studies in soybean for Sclerotinia sclerotiorum resistance reveal multiple modes of resistance in multi-environments." Scientific Reports 7, no. 1 (2017): 3554. doi: 10.1038/s41598-017-03695-9. Posted with permission.