Campus Units

Agronomy

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

6-8-2017

Journal or Book Title

Current Protocols in Plant Biology

First Page

147

Last Page

157

DOI

10.1002/cppb.20050

Abstract

Commercial maize hybrid production has corroborated the usefulness of producing inbred lines; however, the delivery of new lines has always been a major time constraint in breeding programs. Traditional methods for developing inbred lines typically require 6 to 10 generations of self-pollination to obtain sufficient homozygosity. To bypass the time and costs associated with the development of inbred lines, doubled haploid (DH) systems have been widely adopted in the commercial production of maize. Within just two generations, DH systems can create completely homozygous and homogeneous lines. A typical maize DH system, utilizing anthocyanin markers R1-nj or Pl1 for haploid selection, is described in this protocol.

Comments

This is an accepted manuscript of an article published as Vanous, Kimberly, Adam Vanous, Ursula K. Frei, and Thomas Lübberstedt. "Generation of Maize (Zea mays) Doubled Haploids via Traditional Methods." Current Protocols in Plant Biology: 147-157. doi:10.1002/cppb.20050. Posted with permission.

Copyright Owner

John Wiley & Sons, Inc.

Language

en

File Format

application/pdf

Published Version

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